Home > Sandwich ELISA Kits > Mouse Solute Carrier Family 30, Member 1 (SLC30A1) ELISA Kit

Product Name:

Mouse Solute Carrier Family 30, Member 1 (SLC30A1) ELISA Kit

Cat.#:

JL15726

Regulatory Status:

RUO

Alternative:

ZNT1; ZRC1; Zinc Transporter 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.05ng/mL

Specificity:

This kit recognizes Mouse SLC30A1 in samples. No significant cross-reactivity or interference between Mouse SLC30A1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids.

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SLC30A1

Zinc transporter 1 is a protein which is encoded by the SLC30A1 gene.SLC30A1 modulates zinc permeation through the L-type calcium channel. SLC30A1 downregulates not only Zn++ influx, but also Ca++ influx, thereby protecting cells from the effects of excessive cation permeation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.35	1.35	0.8	0.48	0.34	0.26	0.22	0.09
Corrected OD	2.26	1.26	0.71	0.39	0.25	0.17	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse SLC30A1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Mouse SLC30A1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	87-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98